久久精品国产a三级三级三级_依人在线_国产精品18久久久久白浆_男女黄色一级片_久久久精品午夜免费不卡_日本丰满熟妇BBXBBXHD

儀器網(yiqi.com)歡迎您!

| 注冊 登錄
網站首頁-資訊-專題- 微頭條-話題-產品- 品牌庫-搜索-供應商- 展會-招標-采購- 社區-知識-技術-資料庫-方案-產品庫- 視頻

資訊中心

當前位置:儀器網> 資訊中心>巨噬細胞清除操作及文獻實例分析

巨噬細胞清除操作及文獻實例分析

來源:翌圣生物科技(上海)股份有限公司      分類: 2023-08-30 00:00:00 20閱讀次數
掃    碼    分   享

 

01
巨噬細胞清除簡介

巨噬細胞是體內每個器官中唯一都存在的細胞,存在于表皮、角膜和沒有血管的關節內部,其體內生物學研究的重要方法之一是巨噬細胞耗竭。清除巨噬細胞,可以全面了解巨噬細胞在病理條件下的功能。目前,巨噬細胞清除的主要方法包括構建巨噬細胞缺失動物模型和使用藥物(氯膦酸鹽脂質體)去除法,但是,構建巨噬細胞缺失動物模型價格昂貴,周期長,因此,氯膦酸鹽脂質體(Clodronate Liposomes)是目前最為成熟、方便、經濟的一種巨噬細胞清除工具,可以有效的清除動物體內包括肝臟,脾臟,肺部,血液等多個不同組織和部位中的巨噬細胞,也是目前使用最廣泛的一種巨噬細胞清除的方法。

 

02
巨噬細胞清除劑的原理

將氯膦酸鹽包裹進磷脂體的水相中形成氯膦酸鹽脂質體,氯膦酸鹽不能自由的通過脂質體磷脂體雙分子層。注射到生物體內后,氯膦酸鹽脂質體會被巨噬細胞吞噬,在巨噬細胞內的溶酶體磷酸酶的作用下,溶解在脂質體內的氯膦酸鹽會被逐步釋放出來并在細胞內積累,當達到一定濃度時,巨噬細胞將會受到不可逆損傷,誘發細胞凋亡。死亡細胞內的氯膦酸鹽會被釋放到細胞外,隨尿液排出體外。

 

03
巨噬細胞清除劑給藥方法參考

不同種類的小鼠給藥劑量略有差異,可根據以下表格中的給藥劑量摸索。

 

表1.C57小鼠巨噬細胞清除給藥方法

 

04
巨噬細胞標志物

巨噬細胞有很多種亞型,不同的亞型部分標志物不同(見表2),另外,不同組織和器官的巨噬細胞的標志物也會有差異(見表3)。一般在做巨噬細胞清除實驗前,應先查找相關文獻,找出具體清除部位的巨噬細胞的表面標志物,選擇最佳標志物進行流式實驗。

 

表2.不同亞型的巨噬細胞標志物

 

參考文獻:Klopfleisch R. Macrophage reaction against biomaterials in the mouse model - Phenotypes, functions and markers. Acta Biomater. 2016 Oct 1;43:3-13. doi: 10.1016/j.actbio.2016.07.003. Epub 2016 Jul 6. PMID: 27395828.

 

表3.不同組織中巨噬細胞的標志物

 

05
巨噬細胞清除文獻實例

 

文獻一
 
 
 
 
 
 
 
 
 
 
 

小鼠品系:C57BL/6J小鼠

注射方式:靜脈注射

注射劑量:200 μl /只

抗體選擇:F4/80

檢測方法:巨噬細胞耗竭模型采用氯膦酸脂質體,靜脈注射200μL每只小鼠,在注射氯鈉脂質體后24小時,通過流式細胞術檢測巨噬細胞數量。

清除結果:

 

圖1.腹腔注射給藥后巨噬細胞減少

 

參考文獻:Xiong X, Chen S, Shen J, et al. Cannabis suppresses antitumor immunity by inhibiting JAK/STAT signaling in T cells through CNR2. Signal Transduct Target Ther. 2022;7(1):99. Published 2022 Apr 6. doi:10.1038/s41392-022-00918-y(IF:18.187)

 

 

文獻二
 
 
 
 
 
 
 
 
 
 
 

小鼠品系:雌性C57BL/6J小鼠

注射方式:腹腔注射

取樣部位:腹腔灌洗液

注射劑量:第0、3、6天分別給與15 mg/kg巨噬細胞清除劑

抗體選擇:CD11b,F4/80雙陽性

檢測方法:在第0、3、6天將氯膦酸脂質體 (15 mg/kg)或懸浮在200 ml PBS中的空脂質體腹腔注射到6周齡雌性C57BL/6J小鼠體內,以消耗巨噬細胞,并在最后一次脂質體注射后的第3、6天將100萬BMDMs懸浮在100 ml PBS中靜脈注射重建巨噬細胞,然后通過流式細胞術分析小鼠腹腔灌洗液巨噬細胞的損耗和重構。

清除結果:

 

2.注射脂質體后鼠腹腔灌洗液及巨噬細胞重建的流式分析

 

參考文獻:Zhang Z, Chen C, Yang F, et al. Itaconate is a lysosomal inducer that promotes antibacterial innate immunity [published online ahead of print, 2022 May 25. Mol Cell. 2022;S1097-2765(22)00443-9. doi:10.1016/j.molcel.2022.05.009 (IF:17.970)

 

文獻三
 
 
 
 
 
 
 
 
 
 
 

小鼠品系:C57BL/6J

注射方式:靜脈注射

取樣部位:血液、乳腺

注射劑量:200 μl /只

抗體選擇:F4/80

清除方法:巨噬細胞耗竭模型采用氯膦酸脂質體(0.15-0.2 mL/25 g),給與200μL每只小鼠,靜脈注射于哺乳期小鼠體內,消耗血液和乳腺內巨噬細胞,在注射氯鈉脂質體后24、48和72小時,通過流式細胞術檢測乳腺巨噬細胞數量。 

清除結果:

 

圖3.a)血液學自動分析儀檢測血液中單核細胞(血液中巨噬細胞的一種形式)比例的變化;b)流式細胞術檢測乳腺巨噬細胞比例變化

 

參考文獻:Cai J, Peng J, Zang X, Feng J, Li R, Ren P, Zheng B, Wang J, Wang J, Yan M, Liu J, Deng R, Wang D. Mammary Leukocyte-Assisted Nanoparticle Transport Enhances Targeted Milk Trace Mineral Delivery. Adv Sci (Weinh). 2022 Sep;9(26):e2200841. doi: 10.1002/advs.202200841. Epub 2022 Jun 30. PMID: 35773238; PMCID: PMC9475556. (IF:17.521)

 

文獻四
 
 
 
 
 
 
 
 
 
 
 

小鼠品系:C57BL/6

注射方式:腹腔注射

注射劑量:200 μl /只

抗體選擇:F4/80

檢測方法: 第3天腹腔注射氯磷酸脂質體200 μL,去除巨噬細胞。用流式細胞術檢測巨噬細胞缺失效應。

清除結果:

 

圖4.免疫細胞缺失流式分析

 

參考文獻:Yu X, Long Y, Chen B, et al. PD-L1/TLR7 dual-targeting nanobody-drug conjugate mediates potent tumor regression via elevating tumor immunogenicity in a host-expressed PD-L1 bias-dependent way. J Immunother Cancer. 2022;10(10):e004590. doi:10.1136/jitc-2022-004590(IF:12.469)

 

06

產品簡介

產品組成:1 mL脂質體體懸液(1.5-2μm)中所含的氯膦酸大約為5 mg,所用的磷酸鹽緩沖液為10 mM Na2HPO4,10 mM NaH2PO4,140 mM NaCl。

 

07

產品列表

產品名稱

貨號

規格

Clodronate Liposomes(From Vrije Universiteit Amsterdam)體內巨噬細胞清除劑

40337ES08

5 mL

40337ES10

10 mL

Control Liposomes(PBS)體內巨噬細胞清除劑空白脂質體對照

40338ES08

5 mL

40338ES10

10 mL

08

部分參考文獻列表

[1] Liu Z, Gu Y, Chakarov S, et al. Fate Mapping via Ms4a3-Expression History Traces Monocyte-Derived Cells. Cell. 2019;178(6):1509-1525.e19. doi:10.1016/j.cell.2019.08.009(IF:36.216)

[2] Xiong X, Chen S, Shen J, et al. Cannabis suppresses antitumor immunity by inhibiting JAK/STAT signaling in T cells through CNR2. Signal Transduct Target Ther. 2022;7(1):99. Published 2022 Apr 6. doi:10.1038/s41392-022-00918-y(IF:18.187)

[3] Zhang Z, Chen C, Yang F, et al. Itaconate is a lysosomal inducer that promotes antibacterial innate immunity [published online ahead of print, 2022 May 25]. Mol Cell. 2022;S1097-2765(22)00443-9. doi:10.1016/j.molcel.2022.05.009(IF:17.970)

[4] Cai J, Peng J, Zang X, et al. Mammary Leukocyte-Assisted Nanoparticle Transport Enhances Targeted Milk Trace Mineral Delivery [published online ahead of print, 2022 Jun 30]. Adv Sci (Weinh). 2022;e2200841. doi:10.1002/advs.202200841(IF:17.521)

[5] Jin H, Liu K, Tang J, et al. Genetic fate-mapping reveals surface accumulation but not deep organ invasion of pleural and peritoneal cavity macrophages following injury. Nat Commun. 2021;12(1):2863. Published 2021 May 17. doi:10.1038/s41467-021-23197-7(IF:14.919)

[6] Sheng D, Ma W, Zhang R, et al. Ccl3 enhances docetaxel chemosensitivity in breast cancer by triggering proinflammatory macrophage polarization [published correction appears in J Immunother Cancer. 2022 Jun;10(6):]. J Immunother Cancer. 2022;10(5):e003793. doi:10.1136/jitc-2021-003793(IF:13.751)

[7] Yu X, Long Y, Chen B, et al. PD-L1/TLR7 dual-targeting nanobody-drug conjugate mediates potent tumor regression via elevating tumor immunogenicity in a host-expressed PD-L1 bias-dependent way. J Immunother Cancer. 2022;10(10):e004590. doi:10.1136/jitc-2022-004590(IF:12.469)

[8] Zhao L, Zhang H, Liu X, et al. TGR5 deficiency activates antitumor immunity in non-small cell lung cancer via restraining M2 macrophage polarization. Acta Pharm Sin B. 2022;12(2):787-800. doi:10.1016/j.apsb.2021.07.011(IF:11.614)

[9] Xia L, Zhang C, Lv N, et al. AdMSC-derived exosomes alleviate acute lung injury via transferring mitochondrial component to improve homeostasis of alveolar macrophages. Theranostics. 2022;12(6):2928-2947. Published 2022 Mar 21. doi:10.7150/thno.69533(IF:11.556)

[10] Zhang X, Hou L, Li F, et al. Piezo1-mediated mechanosensation in bone marrow macrophages promotes vascular niche regeneration after irradiation injury. Theranostics. 2022;12(4):1621-1638. Published 2022 Jan 16. doi:10.7150/thno.64963(IF:11.556)

[11] Xun J, Du L, Gao R, et al. Cancer-derived exosomal miR-138-5p modulates polarization of tumor-associated macrophages through inhibition of KDM6B. Theranostics. 2021;11(14):6847-6859. Published 2021 May 3. doi:10.7150/thno.51864(IF:11.556)

[12] Wang H, Li L, Li Y, et al. Intravital imaging of interactions between iNKT and kupffer cells to clear free lipids during steatohepatitis. Theranostics. 2021;11(5):2149-2169. Published 2021 Jan 1. doi:10.7150/thno.51369(IF:11.556)

[13] Wu H, Yin X, Zhao X, et al. HDAC11 negatively regulates antifungal immunity by inhibiting Nos2 expression via binding with transcriptional repressor STAT3. Redox Biol. 2022;56:102461. doi:10.1016/j.redox.2022.102461(IF:10.787)

[14] Liu X, Fei H, Yang C, et al. Trophoblast-Derived Extracellular Vesicles Promote Preeclampsia by Regulating Macrophage Polarization. Hypertension. 2022;79(10):2274-2287. doi:10.1161/HYPERTENSIONAHA.122.19244(IF:9.897)

[15] Zuo L, Li J, Zhang X, et al. Aberrant mesenteric adipose extracellular matrix remodeling is involved in adipocyte dysfunction in Crohn's disease: The role of TLR-4-mediated macrophages [published online ahead of print, 2022 Jun 16]. J Crohns Colitis. 2022;jjac087. doi:10.1093/ecco-jcc/jjac087(IF:9.071)

[16] Huang C, Wang J, Liu H, et al. Ketone body β-hydroxybutyrate ameliorates colitis by promoting M2 macrophage polarization through the STAT6-dependent signaling pathway. BMC Med. 2022;20(1):148. Published 2022 Apr 15. doi:10.1186/s12916-022-02352-x(IF:8.775)

[17] Wang Y, Sun Q, Ye Y, et al. FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis. JCI Insight. 2022;7(10):e157874. Published 2022 May 23. doi:10.1172/jci.insight.157874(IF:8.315)

[18] Jiang P, Gao W, Ma T, et al. CD137 promotes bone metastasis of breast cancer by enhancing the migration and osteoclast differentiation of monocytes/macrophages. Theranostics. 2019;9(10):2950-2966. Published 2019 May 9. doi:10.7150/thno.29617(IF:8.063)

[19] Yang XL, Wang G, Xie JY, et al. The Intestinal Microbiome Primes Host Innate Immunity against Enteric Virus Systemic Infection through Type I Interferon. mBio. 2021;12(3):e00366-21. Published 2021 May 11. doi:10.1128/mBio.00366-21(IF:7.867)

[20] Li C, Song J, Guo Z, et al. EZH2 Inhibitors Suppress Colorectal Cancer by Regulating Macrophage Polarization in the Tumor Microenvironment. Front Immunol. 2022;13:857808. Published 2022 Apr 1. doi:10.3389/fimmu.2022.857808(IF:7.561)

[21] Sun Z, Huang W, Zheng Y, et al. Fpr2/CXCL1/2 Controls Rapid Neutrophil Infiltration to Inhibit Streptococcus agalactiae Infection. Front Immunol. 2021;12:786602. Published 2021 Nov 24. doi:10.3389/fimmu.2021.786602(IF:7.561)

[22] Cai J, Cui X, Wang X, You L, Ji C, Cao Y. A Novel Anti-Infective Peptide BCCY-1 With Immunomodulatory Activities. Front Immunol. 2021;12:713960. Published 2021 Jul 22. doi:10.3389/fimmu.2021.713960(IF:7.561)

[23] Chen X, Zheng Y, Liu S, Yu W, Liu Z. CD169<sup>+</sup> subcapsular sinus macrophage-derived microvesicles are associated with light zone follicular dendritic cells. Eur J Immunol. 2022;52(10):1581-1594. doi:10.1002/eji.202249879(IF:6.688)

[24] Tian L, Li W, Yang L, et al. Cannabinoid Receptor 1 Participates in Liver Inflammation by Promoting M1 Macrophage Polarization via RhoA/NF-κB p65 and ERK1/2 Pathways, Respectively, in Mouse Liver Fibrogenesis. Front Immunol. 2017;8:1214. Published 2017 Sep 28. doi:10.3389/fimmu.2017.01214(IF:6.429)

[25] Zou D, Qin J, Hu W, et al. Macrophages Rapidly Seal off the Punctured Zebrafish Larval Brain through a Vital Honeycomb Network Structure. Int J Mol Sci. 2022;23(18):10551. Published 2022 Sep 11. doi:10.3390/ijms231810551(IF:6.208)

[26] Wang X, Li W, Jiang H, et al. Zebrafish Xenograft Model for Studying Pancreatic Cancer-Instructed Innate Immune Microenvironment. Int J Mol Sci. 2022;23(12):6442. Published 2022 Jun 9. doi:10.3390/ijms23126442(IF:5.924)

[27] Xiong C, Zhu Y, Xue M, et al. Tumor-associated macrophages promote pancreatic ductal adenocarcinoma progression by inducing epithelial-to-mesenchymal transition. Aging (Albany NY). 2021;13(3):3386-3404. doi:10.18632/aging.202264(IF:5.682)

[28] Pu S, Wu Y, Tong F, et al. Mechanosensitive Ion Channel TMEM63A Gangs Up with Local Macrophages to Modulate Chronic Post-amputation Pain [published online ahead of print, 2022 Jul 12]. Neurosci Bull. 2022;10.1007/s12264-022-00910-0. doi:10.1007/s12264-022-00910-0(IF:5.271)

[29] Wang J, Li X, Wang Y, Li Y, Shi F, Diao H. Osteopontin aggravates acute lung injury in influenza virus infection by promoting macrophages necroptosis. Cell Death Discov. 2022;8(1):97. Published 2022 Mar 4. doi:10.1038/s41420-022-00904-x(IF:5.241)

[30] Ma Y, Liang Y, Wang N, et al. Avian Flavivirus Infection of Monocytes/Macrophages by Extensive Subversion of Host Antiviral Innate Immune Responses. J Virol. 2019;93(22):e00978-19. Published 2019 Oct 29. doi:10.1128/JVI.00978-19(IF:5.103)

[31] Jiang Q, Li W, Zhu X, et al. Estrogen receptor β alleviates inflammatory lesions in a rat model of inflammatory bowel disease via down-regulating P2X7R expression in macrophages. Int J Biochem Cell Biol. 2021;139:106068. doi:10.1016/j.biocel.2021.106068(IF:5.085)

[32] Tang Y, Wang C, Chen S, et al. Dimethyl fumarate attenuates LPS induced septic acute kidney injury by suppression of NFκB p65 phosphorylation and macrophage activation. Int Immunopharmacol. 2022;102:108395. doi:10.1016/j.intimp.2021.108395(IF:4.932)

[33] Lu Y, Lu G, Gao L, et al. The Proresolving Lipid Mediator Maresin1 Alleviates Experimental Pancreatitis via Switching Macrophage Polarization. Mediators Inflamm. 2021;2021:6680456. Published 2021 Mar 9. doi:10.1155/2021/6680456(IF:4.711)

[34] Li W, Chang N, Tian L, et al. miR-27b-3p, miR-181a-1-3p, and miR-326-5p are involved in the inhibition of macrophage activation in chronic liver injury. J Mol Med (Berl). 2017;95(10):1091-1105. doi:10.1007/s00109-017-1570-0(IF:4.686)

[35] Ji L, Chen Y, Xie L, Liu Z. The role of Dock2 on macrophage migration and functions during Citrobacter rodentium infection. Clin Exp Immunol. 2021;204(3):361-372. doi:10.1111/cei.13590(IF:4.330)

[36] Yang L, Dong C, Tian L, Ji X, Yang L, Li L. Gadolinium Chloride Restores the Function of the Gap Junctional Intercellular Communication between Hepatocytes in a Liver Injury. Int J Mol Sci. 2019;20(15):3748. Published 2019 Jul 31. doi:10.3390/ijms20153748(IF:4.183)

[37] Wu H, Xu X, Li J, Gong J, Li M. TIM?4 blockade of KCs combined with exogenous TGF?β injection helps to reverse acute rejection and prolong the survival rate of mice receiving liver allografts. Int J Mol Med. 2018;42(1):346-358. doi:10.3892/ijmm.2018.3606(IF:4.101)

[38] Zhao J, Chen XD, Yan ZZ, Huang WF, Liu KX, Li C. Gut-Derived Exosomes Induce Liver Injury After Intestinal Ischemia/Reperfusion by Promoting Hepatic Macrophage Polarization [published online ahead of print, 2022 Jun 14]. Inflammation. 2022;10.1007/s10753-022-01695-0. doi:10.1007/s10753-022-01695-0(IF:4.092)

[39] Guan Z, Ding Y, Liu Y, et al. Extracellular gp96 is a crucial mediator for driving immune hyperactivation and liver damage. Sci Rep. 2020;10(1):12596. Published 2020 Jul 28. doi:10.1038/s41598-020-69517-7(IF:3.998)

[40] Song C, Li H, Li Y, et al. NETs promote ALI/ARDS inflammation by regulating alveolar macrophage polarization. Exp Cell Res. 2019;382(2):111486. doi:10.1016/j.yexcr.2019.06.031(IF:3.329)

[41] Li R, Yang L, Jiang N, et al. Activated macrophages are crucial during acute PM2.5 exposure-induced angiogenesis in lung cancer. Oncol Lett. 2020;19(1):725-734. doi:10.3892/ol.2019.11133(IF:1.871)

<上下滑動查看更多>

 

 

參與評論

全部評論(0條)

獲取驗證碼
我已經閱讀并接受《儀器網服務協議》

推薦閱讀

版權與免責聲明

①本文由儀器網入駐的作者或注冊的會員撰寫并發布,觀點僅代表作者本人,不代表儀器網立場。若內容侵犯到您的合法權益,請及時告訴,我們立即通知作者,并馬上刪除。

②凡本網注明"來源:儀器網"的所有作品,版權均屬于儀器網,轉載時須經本網同意,并請注明儀器網(www.ghhbs.com.cn)。

③本網轉載并注明來源的作品,目的在于傳遞更多信息,并不代表本網贊同其觀點或證實其內容的真實性,不承擔此類作品侵權行為的直接責任及連帶責任。其他媒體、網站或個人從本網轉載時,必須保留本網注明的作品來源,并自負版權等法律責任。

④若本站內容侵犯到您的合法權益,請及時告訴,我們馬上修改或刪除。郵箱:hezou_yiqi

關于作者

作者簡介:翌圣生物科技(上海)股份有限公司是一家聚焦生命科學產業鏈上游核心原料,從事分子、蛋白和細胞三...[詳細]
最近更新:2024-12-18 14:04:41
關注 私信
更多

最新話題

最新資訊

作者榜

主站蜘蛛池模板: 车上震动A级作爱视频 | 日产精品卡一卡2卡三卡四 日韩欧美永久中文字幕视频 | 狠狠躁夜夜躁人人爽天天5 国产精品宾馆在线精品酒店 | 亚洲性色av一区二区三区 | 成人性生活免费看 | 日本动漫瀑乳H动漫啪啪免费 | 国产99视频精品免费观看9 | 久久成人18免费网站 | 天下免费大全正版资料 | 婷婷中文字幕一区 | 久久这里只精品国产免费99热4 | 午夜影视av | 国产精品高清视亚洲精品 | 一级视频在线观看免费 | 亚洲精品久久久久久久久久久久久 | 综合成人在线 | 日韩成人高清视频 | 人妻换着玩又刺激又爽 | 国产7座suv精品麻豆啪最新 | 精品国产91亚洲一区二区三区婷婷 | 好吊妞国产欧美日韩免费观看视频 | 日产乱码一二三区别免费麻豆 | 最近中文视频在线 | 天天色天天拍 | 亚洲日本无码AA在线播放 | 久久精品国产亚洲77788 | 91视频免费高清 | 日本中文字幕在 | 日韩欧美2区 | 四川一级淫片a按摩店 | 国产农村妇女三级全黄91 | 国产一区二区三区在线观看视频 | 亚洲性无码AV在线观看 | 国产成年人小视频 | 艳妇乳肉豪妇荡乳流白浆 | 激情综合婷婷丁香五月 | 免费观看久久 | 亚洲无线码高清在线观看 | 亚洲国产精品国自产拍av绿帽子 | 国产强伦姧在线观看无码 | 国产精品原创巨作AV女教师 |