久久精品国产a三级三级三级_依人在线_国产精品18久久久久白浆_男女黄色一级片_久久久精品午夜免费不卡_日本丰满熟妇BBXBBXHD

儀器網（yiqi.com）歡迎您！

| 注冊 登錄
網站首頁-資訊-專題- 微頭條-話題-產品- 品牌庫-搜索-供應商- 展會-招標-采購- 社區-知識-技術-資料庫-方案-產品庫- 視頻

產品中心

當前位置：儀器網>產品中心> 廣州安邦生物科技有限公司>生物試劑>抗體>Human HMGB1 recombinant protein (Active) (His-tagg
收藏  

Human HMGB1 recombinant protein (Active) (His-tagg

立即掃碼咨詢

聯系方式：400-822-6768

聯系我們時請說明在儀器網（www.ghhbs.com.cn）上看到的!

掃    碼    分   享
為您推薦

產品特點

HMGB1 是一種多功能蛋白，在炎癥與腫瘤均扮演重要角色。HMGB1 可做為損傷相關分子模式 (DAMP) 分子誘發炎癥；然而過量 HMGB1 所誘發的高強度炎癥反而會導致機體組織損傷。另外，腫瘤細胞分泌的 HMGB1 可促進血管生成與免疫抑制，從而推進腫瘤進展。

詳細介紹

概述

產品描述E. coli expressed, His-tagged (C-ter) Active Human HMGB1 recombinant protein.
反應物種Hu, Ms
應用FuncSt, SDS-PAGE
靶點名稱HMGB1
物種Human
A.A. 序列MGKGD PKKPR GKMSS YAFFV QTCRE EHKKK HPDAS VNFSE FSKKC SERWK TMSAK EKGKF EDMAK ADKAR YEREM KTYIP PKGET KKKFK DPNAP KRPPS AFFLF CSEYR PKIKG EHPGL SIGDV AKKLG EMWNN TAADD KQPYE KKAAK LKEKY EKDIA AYRAK GKPDA AKKGV VKAEK SKKKK EEEED EEDEE DEEEE EDEED EDEEE DDDDE with polyhistidine tag at the C-terminus.
表達系統E. coli
活動Active
活動說明Measure by its ability to induce TNF alpha in Raw264.7 cells.
for this effect is < 10 μg/ml. This protein also induces TNF alpha releasing from human A549 cultured cells based on customer feedback.
別名HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

屬性

形式Powder
純化Ni-NTA chromatography.
純化說明Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
純度> 98% (by SDS-PAGE)
緩沖液PBS (pH 8.0)
重建It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
存放說明Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
注意事項For laboratory research only, not for drug, diagnostic or other use.

生物信息

數據庫連接

 GeneID: 15289 Mouse HMGB1

 GeneID: 3146 Human HMGB1

 Swiss-port # P09429 Human High mobility group protein B1

 Swiss-port # P63158 Mouse High mobility group protein B1

基因名稱HMGB1
全名high mobility group box 1
背景介紹HMGB1 is a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
生物功能HMGB1 is a DNA binding protein. It associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). [UniProt]
細胞定位Nucleus. Chromosome. Cytoplasm. Secreted. Cell membrane; Peripheral membrane protein; Extracellular side. Endosome. Endoplasmic reticulum-Golgi intermediate compartment. Note=In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus. Translocates from the nucleus to the cytoplasm upon autophagy stimulation. Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes. [UniProt]
產品亮點Related products:
HMGB1 antibodies; HMGB1 ELISA Kits; HMGB1 Duos / Panels; HMGB1 recombinant proteins;
Related news:
HMGB1, a biomarker and therapeutic target in COVID-19
Total solution for HMGB1 research
HMGB1 in inflammation
Inflammatory Cytokines
HMGB1 ELISA Kit for your research
Detecting the DAMPs in cancer therapy by HMGB1 ELISA kit
New HMGB1 neutralizing antibody is released
Detecting exosomal HMGB1 for ICD research
Related poster download:
HMGB1 Pathway.pdf
預測分子量25 kDa
翻譯后修飾Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460).

Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity).

Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so).

Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity).

In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex; reduces binding to heparin and proinflammatory activities (By similarity). [UniProt]


廠商推薦產品

在線留言

換一張？
取消
主站蜘蛛池模板： www.玖玖玖| 欧美色倩网站大全免费 | 亚洲精品久久久久久久久久久久 | 韩日一级片 | 色综合精品久久久久久久 | 色爱天堂 | 一级片久久免费 | 成人无遮挡裸免费视频在线观看 | 和小三爽到喷水h | 欧美裸体XXXX极品少妇 | 国产韩国欧美在线不卡 | 亚洲天堂男人影院 | 免费黄色大片网站 | 一区二区三区视频免费视频观看网站 | 刘亦菲毛片一区二区三区 | 国产播放隔着超薄丝袜进入 | 国产精品国产三级国产AV中文 | 裸体黑色丝袜18禁网站无风险 | 天天夜操 | 亚洲观看在线 | 99热新 | 国产精品久久不射 | 99久久久久久99国产精品免 | 超碰caoporm| 新版天堂网www天堂在线资源 | 日韩在线播放视频 | 9久久9毛片又大又硬又粗 | 99精品人妻少妇一区二区 | 日韩爱爱| 免费看黄色一级 | 卡一卡二卡三免费视频 | 在线免费看av的网站 | 国产乱子轮XXX农村 婷婷五月综合国产激情 | 久久在草 | youyou交视频 | 成人精品小视频 | 国产精品美女视频 | 欧美噜噜久久久xxx成人高潮 | 99久久综合网 | 亚洲日韩国产二区无码 | 国产视频一区二区在线播放 |